Sleep and Stress Panel

Test Details

Lab Company: Ayumetrix
Sample Type: Saliva
Collection Location:  At Home

The Sleep and Stress Panel measures melatonin and cortisol at four points throughout the day to determine hormone fluctuations and imbalances.        

🔑 Key Details 📦 Shipping
Preparation & Timing 📊 Results
🧪 Collection Instructions 📚 More Resources
💳 Requisition & Payment
💬 Questions?

Key Details 

  • If you are a male or non-menstruating female, collect your sample on any day.
  • If you are menstruating female, collect your sample according to the schedule below. 
  • Do not collect your samples if you are sick. 
  • Do not drink alcohol on the day of sample collection. 
  • If you are taking hormones, you may need to time your test appropriately around your doses. 
  • You will collect four saliva samples. 
  • Ship samples via UPS the day after collection.

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Preparation & Timing

  • If you are a male or non-menstruating female, collect your sample on any day.
  • If you are menstruating female, 
    • Regular cycles: Collect samples between days 19-21 of your cycle (where day 1 is the first day of your period).
    • Irregular cycles: Collect samples any day you are not bleeding. 
    • Find more information here.
  • Do not collect your samples if you are sick. 
  • Avoid any over-the-counter anti-aging creams for 3 days prior to sample collection. 
  • Do not drink alcohol on the day of sample collection. 
  • Do not eat, drink, brush your teeth, or exercise prior to your first saliva collection. 

Hormone Users

  • Avoid taking 7-Keto-DHEA for 3 days prior to sample collection. 
  • If you use topical hormones, 
    • Avoid applying hormones to your face or neck for 3 days prior to sample collection. 
    • Avoid applying hormones using your fingers for 2 days prior to sample collection. 
    • Avoid applying hormones to exposed parts of your body.
    • Wash your hands thoroughly with soap and water after applying hormones.
    • Do not use the day of collection. 
    • Collect your samples 12-24 hours after your last dose.
  • If you use sublingual hormones or troches, 
    • Collect your samples 24-36 hours after your last dose.
    • Do not use the day of collection. 
  • If you use oral hormones, 
    • Progesterone: Collect your samples 6-10 hours after your last dose.
    • All other hormones: Collect your samples 12-24 hours after your last dose.
  • If you use hormone injections, pellets, or transdermal patches, 
    • Collect your samples halfway between doses. 
  • If you are taking cortisol supplementation, 
    • Talk to your healthcare provider about timing if you are taking cortisol for adrenal support. 
    • Avoid cortisol-containing products for 5 days prior to sample collection.
  • You can continue taking any other hormones, supplements, or vitamins, but please note them on your test requisition form. 

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Collection Instructions

Collection Schedule

  1. Collection 1: Within 30 minutes of waking
    1. Collect prior to eating, drinking, brushing your teeth, smoking, using tobacco, or exercising. 
  2. Collection 2: Mid-day (around 12 pm)
    1. Do not eat a big meal within 60 minutes of sample collection. 
    2. Do not smoke, use tobacco, drink anything, or brush your teeth for 30 minutes prior to sample collection. 
    3. Do not eat dairy products, acidic foods, or sugary foods for 30 minutes prior to sample collection. 
  3. Collection 3: Afternoon (around 4 pm)
    1. Do not eat a big meal within 60 minutes of sample collection. 
    2. Do not smoke, use tobacco, drink anything, or brush your teeth for 30 minutes prior to sample collection. 
    3. Do not eat dairy products, acidic foods, or sugary foods for 30 minutes prior to sample collection. 
  4. Collection 4: At bedtime 
    1. Do not eat a big meal within 60 minutes of sample collection.
    2. Do not smoke, use tobacco, drink anything, or brush your teeth for 30 minutes prior to sample collection. 
    3. Do not eat dairy products, acidic foods, or sugary foods for 30 minutes prior to sample collection. 

Collection Instructions

  1. Wash your hands and dry with a clean towel.
  2. Open the kit and get only one collection tube.
  3. Seal kit immediately.
  4. Unscrew the cap on the tube.
  5. Pool saliva in your mouth.
    1. It may help to think about your favorite food. 
  6. Tilt your head forward and push saliva down into the tube using your tongue
  7. Fill the tube at least 3/4 full (excluding bubbles). 
  8. Screw the cap tightly on the tube.
  9. Write your name, the date of collection, and the time of collection on the tube.
  10. Place the sample tube inside the ziplock bag.
  11. Place the ziplock bag into the freezer. 
    1. Each sample tube will be added to this bag. 
    2. Samples will stay in the freezer until shipped.
  12. Fill in the date and time of collection for that sample in section 7 of the test requisition form. 
  13. Repeat steps 1-12 for each sample collection. 

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Requisition & Payment

  • Your kit has been prepaid. You DO NOT need to pay anything extra.
  • Instructions on how to fill out your requisition can be found here.
    • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.

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Shipping

  • Ship samples the day after collection (or as soon as possible).
  • Ship via UPS.
  • Ship using the provided materials.
    • Remove the ziplock bag containing the saliva samples from the freezer. 
    • Place the ziplock bag into the kit box.
    • Place the completed test requisition form into the kit box. 
    • Place the kit into the provided return mailer. 
    • Place the prepaid shipping label on the mailer. 

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Results

  • Results should be released to your provider 3-5 business days after samples are received at the lab. We cannot guarantee processing time. 
  • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 
  • Your provider may choose to release your results to you. Rupa cannot release your results without provider authorization.

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More Resources

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Questions?

For general questions, email Rupa Health at support@rupahealth.com. For test-specific questions, contact Ayumetrix by calling 800-215-8898 or chatting with support