Cortisol Profile

Test Details

Lab Company: Sanesco & NeuroLab
Sample Type: Saliva
Collection Location: At Home

The Cortisol Profile assesses cortisol levels throughout the day.    

🔑 Key Details 📦 Shipping
Preparation & Timing 📊 Results
🧪 Collection Instructions 📚 More Resources
💳 Requisition & Payment
💬 Questions?

Key Details 

  • Collect all of your samples on the same day. 
  • Ship your samples via FedEx within 7 days of collection. 
  • Avoid alcohol, topical hormones, and strenuous exercise on the day of collection.

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Preparation & Timing

  • The day before collection
    • Avoid alcohol for at least 12 hours prior to the start of sample collection.
  • The day of collection
    • Avoid applying topical hormones.
    • Avoid alcohol.
    • Avoid strenuous exercise.
    • Avoid napping for 90 minutes or longer.
    • Do not eat, chew gum, smoke, brush your teeth, or drink anything but water for at least 1 hour prior to each saliva collection.

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Collection Instructions

Collection Schedule

You will collect four saliva collections throughout the day. Proper timing is important.     

  1. Collection 1 - GREEN tube
    1. 1 hour after waking
  2. Collection 2 - RED tube
    1. Before lunch
    2. Collection 2 must be 4-5 hours after Collection 1.
  3. Collection 3 - YELLOW tube
    1. Before dinner
    2. Collection 3 must be 4-5 hours after Collection 2.
  4. Collection 4 - BLUE tube
    1. Before bed
    2. Collection 4 must be 4-5 hours after Collection 3.

    Collection Instructions

    1. Do not eat, chew gum, smoke, brush your teeth, or drink anything but water for at least 1 hour prior to each saliva collection.
    2. Rinse your mouth with warm water. 
    3. Wait 10 minutes before starting your collection. 
    4. Open the appropriate tube (see schedule and tube colors above). 
    5. Pool saliva in your mouth. 
      1. If you have trouble producing saliva, try
        1. Thinking about your favorite food.
        2. Yawning. 
        3. Smelling lemons or lemon juice. 
    6. Carefully spit saliva into the tube. 
    7. Fill the tube to the fill line (not including bubbles). 
      1. The liquid part of your sample should reach the fill line.
    8. Close the tube tightly.
    9. Place the tube in the freezer immediately. 
      1. The sample should be frozen for a minimum of 4 hours prior to shipping.
    10. Repeat steps 1-9 for each of the four saliva collections. 

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    Requisition & Payment

    • Your kit has been prepaid. You DO NOT need to pay anything extra.
    • Instructions on how to fill out your requisition can be found HERE.
      • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.

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    • Samples should be shipped within 7 days of collection. 
    • To prepare your samples for shipping, 
      • Place the completed test requisition form into the outside compartment of the specimen bag. 
      • Place the four saliva sample tubes in the specimen bag.
        • Do NOT remove the absorbent sheet. 
      • Seal the specimen bag. 
      • Place the specimen bag in the white shipping box. 
        • Do not include any ice packs. 
      • Seal the white shipping box. 
      • Place the prepaid shipping label on the box. 
    • Make a note of the tracking number so that you can track your samples back to the lab. 
    • You can take your samples to the nearest FedEx location for them to be shipped back to the lab.

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    • Results should be released to your provider 7-10 business days after samples are received at the lab. We cannot guarantee processing time. 
    • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 
    • Your provider may choose to release your results to you. Rupa cannot release your results without provider authorization.

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    More Resources

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    For general questions, email Rupa Health at For test-specific questions, contact Sanesco by calling 866-670-5705 or emailing