Hormone Trio Saliva

Test Details

Lab Company: Ayumetrix
Sample Type: Saliva
Collection Location:  At Home

The Hormone Trio provides a baseline assessment of estradiol, progesterone, and testosterone. These hormones play a significant role in both men's and women's overall health.         

🔑 Key Details 📦 Shipping
Preparation & Timing 📊 Results
🧪 Collection Instructions 📚 More Resources
💳 Requisition & Payment
💬 Questions?

Key Details 

  • If you are menstruating female, collect your sample according to the schedule below. 
  • If you are using hormones, collect your sample according to the schedule below. 
  • You must avoid certain medications and activities on the day of sample collection.
  • You will collect only one sample - in the morning, upon waking. 
  • Ship your sample as soon as possible

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Preparation & Timing

  • If you are a male or non-menstruating female, collect your sample on any day.
    • If you are taking hormones, see below for timing information.
  • If you are menstruating female, 
    • Regular cycles: Collect your sample between days 19-21 of your cycle (where day 1 is the first day of your period).
    • Irregular cycles: Collect your sample any day you are not bleeding. 
    • Find more information HERE.
  • Avoid the following for 3 days prior to sample collection. 
    • 7-Keto-DHEA
    • Over-the-counter anti-aging creams 
  • If you use topical hormones, 
    • Avoid applying hormones to your face or neck for 3 days prior to sample collection. 
    • Avoid applying hormones using your fingers for 2 days prior to sample collection. 
    • Avoid applying hormones to exposed parts of your body.
    • Wash your hands thoroughly with soap and water after applying hormones.
    • Collect your samples 12-24 hours after your last dose.
    • Do not use topical hormones on the day of collection.
  • If you use sublingual hormones or troches, 
    • Collect your samples 24-36 hours after your last dose.
    • Do not use sublingual hormones or troches on the day of collection.
  • If you use oral hormones, 
    • Progesterone: Collect your samples 6-10 hours after your last dose.
    • All other hormones: Collect your samples 12-24 hours after your last dose.
  • If you use hormone injections or pellets, 
    • Collect your samples halfway between doses. 
  • If you use transdermal patches, 
    • Collect your samples 1-2 days after applying the patch. 
  • You can continue taking any other hormones, supplements, or vitamins, but please note them on your test requisition form.
  • On the day of collection, avoid: 
    • Taking topical hormones
    • Taking sublingual hormones or troches
    • Drinking alcoholic beverages
    • Using lip products
  • For 30 minutes prior to collection, avoid: 
    • Smoking
    • Using tobacco
    • Drinking anything
    • Eating anything 
    • Brushing your teeth

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Collection Instructions

  1. You will collect your sample in the morning, immediately upon waking. 
  2. Wash your hands and dry with a clean towel.
  3. Open the kit and get only one collection tube.
    1. You will only collect ONE sample. 
  4. Seal kit immediately.
  5. Unscrew the cap on the tube.
  6. Pool saliva in your mouth.
    1. If you're having trouble producing saliva, try
      1. Thinking about your favorite food
      2. Smelling food
      3. Yawning
  7. Tilt your head forward and push saliva down into the tube using your tongue
  8. Fill the tube at least 3/4 full (excluding bubbles). 
  9. Screw the cap tightly on the tube.
  10. Write your name, the date of collection, and the time of collection on the tube.
  11. Place the sample tube inside the ziplock bag.
  12. Place the ziplock bag into the freezer. 
    1. Keep your sample in the freezer until shipped.

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Requisition & Payment

  • Your kit has been prepaid. You DO NOT need to pay anything extra.
  • Instructions on how to fill out your requisition can be found HERE.
    • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.

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  • Ship samples as soon as possible after collection. 
  • To prepare your samples for shipping, 
    • Remove the ziplock bag containing the saliva sample from the freezer. 
    • Place the ziplock bag into the kit box.
    • Place the completed test requisition form into the kit box. 
    • Place the kit into the provided return envelope. 
    • Place the prepaid shipping label on the evelope. 
  • Make a note of the tracking number so that you can track your samples back to the lab.

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  • Results should be released to your provider 3-5 business days after samples are received at the lab. We cannot guarantee processing time. 
  • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 
  • Your provider may choose to release your results to you. Rupa cannot release your results without provider authorization.

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More Resources

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For general questions, email Rupa Health at support@rupahealth.com. For test-specific questions, contact Ayumetrix by calling 800-215-8898 or chatting with support