Melatonin

Preparation & Timing

• You will collect FOUR saliva samples. ALL SAMPLES MUST BE COLLECTED ON THE SAME DAY.
• If you are a male or non-menstruating female, you can collect your samples on any day.
• If you are menstruating female, 
  • Regular cycles: Collect samples between days 19-21 of your cycle (where day 1 is the first day of your period).
  • Irregular cycles: Collect samples any day you are not bleeding. 
  • Find more information on timing your test properly HERE.
• DO NOT collect your samples if you are sick. 
• You can continue to take most of your supplements and vitamins. Please note any supplements you take on collection day on the requisition form. 
• If you take hormones, please see the timing information below.
• 3 days prior to sample collection
  • DO NOT use any over-the-counter anti-aging creams. 
• On the day of sample collection, 
  • DO NOT drink alcohol. 
  • DO NOT use any lip products (lipstick, chapstick, etc.).
  • DO NOT use hormones.
• Within 60 minutes of each sample collection, 
  • DO NOT eat a big meal. 
• Within 30 minutes of each sample collection,
  • DO NOT eat.
  • DO NOT drink anything but water.
  • DO NOT brush your teeth.
  • DO NOT exercise. 
  • DO NOT smoke or use tobacco products.

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Collection Instructions

Collection Schedule

1. Collection 1: Within 30 minutes of waking
2. Collection 2: Noon (around 12 pm)
3. Collection 3: Afternoon (around 4 pm)
4. Collection 4: At bedtime 

Collection Instructions

1. Rinse your mouth with water 5 minutes prior to sample collection.
2. Wash your hands and dry with a clean towel.
3. Open the kit and get only one collection tube.
4. Close the kit immediately.
5. Unscrew the cap on the tube.
6. Pool saliva in your mouth.
   1. TIP: It may help to yawn or think about your favorite food. 
7. Tilt your head forward. 
8. Push saliva down into the tube using your tongue. 
9. Fill the tube at least 3/4 full (excluding bubbles). 
10. Screw the cap tightly on the tube.
11. Write the following information on the tube.
    1. Full name
    2. Date of collection
    3. Time of collection
12. Place the sample tube inside the ziplock bag.
13. Close the ziplock bag tightly. 
14. Place the ziplock bag into the freezer. 
    1. Each sample tube will be added to this bag. 
    2. Samples will stay in the freezer until shipped.
15. Write the following information in section 7 of the test requisition form. 
    1. Date of collection
    2. Time of collection
16. Repeat steps 1-15 for each sample collection. 

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Requisition & Payment

• Your kit has been prepaid. You DO NOT need to pay anything extra.
• Instructions on how to fill out your requisition can be found HERE.
  • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.

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Shipping

• Ship samples the day after collection (or as soon as possible).
• To prepare your samples for shipping,
  • Remove the ziplock bag containing the saliva samples from the freezer. 
  • Place the ziplock bag into the test kit box.
  • Place the completed test requisition form into the test kit box. 
  • Place the test kit box into the provided return mailer. 
  • Place the prepaid shipping label on the mailer. 
• Make a note of the return tracking number on the label so you can track your samples back to the lab. 

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Results

• Results should be released to your provider 3-5 business days after samples are received at the lab. We cannot guarantee processing time. 
• Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 

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More Resources

• Melatonin Info Page
• Ayumetrix Patient FAQ
• [VIDEO] How to Collect Your Sample

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Questions?

For general questions, email Rupa Health at support@rupahealth.com. For test-specific questions, contact Ayumetrix by calling 800-215-8898 or chatting with support.