Lyme Dot Blot Assay (LDA) – 2 samples

Test Details

Lab Company: IGeneX
Sample Type: Urine
Collection Location: At Home

The Lyme Dot Blot Assay detects B. burgdorferi infection through a urine sample.    

🔑 Key Details 📦 Shipping
Preparation & Timing 📊 Results
🧪 Collection Instructions 📚 More Resources
💳 Requisition & Payment
💬 Questions?

Key Details 

  • DO NOT collect while menstruating.
  • You must collect your first morning urination.
  • You will collect your samples on 2 consecutive days.
  • Keep samples in the refrigerator until they are shipped.
  • Ship using FedEx as soon as possible after collection. 

back to top

Preparation & Timing

  • DO NOT collect while menstruating. 
  • You must collect your first morning urination.
  • You will collect your samples on 2 consecutive days.

back to top

Collection Instructions

Collection Schedule

When to Collect # of Tubes
Day 1 Upon waking 2 tubes
Day 2 Upon waking 2 tubes

Collection Instructions

  1. Wash your hands with soap and warm water. 
  2. Dry your hands with a clean paper towel or allow them to air dry.
  3. Take the urine collection cup out of the test kit. 
    1. DO NOT touch the inside of the cup.
  4. Open the towelette and wipe yourself.
    1. If you are female, separate your labia with your fingers and clean inside with the towelette, moving from front to back. 
    2. If you are male, wipe the end of your penis with the towelette.
  5. Allow a small amount of urine to enter the toilet to eliminate any contaminants. 
  6. Collect your mid-stream urination in the collection cup until it is filled.
  7. Pass any remaining urine into the toilet.
  8. Open one of the gray-top tubes.
  9. Use the provided pipette to transfer urine from the collection cup to the gray-top tube.
  10. Fill the tube past the minimum fill line (indicated on the label).
  11. Place the pipette back into the urine collection cup.
  12. Cap the tube tightly. 
  13. Gently invert the tube 8-10 times to mix the preservative with the specimen. 
  14. Repeat steps 8-13 for the second tube.
  15. Write the following information on two of the white tube labels.  
    1. Full name
    2. Date of birth
    3. Date of collection
  16. Place the labels on the 2 gray-top tubes. 
  17. Discard any remaining urine.
  18. Place the 2 gray-top tubes into the white tube holder.
  19. Place the tube holder into the biohazard ziplock bag and seal.
  20. Place the ziplock bag into the bubble pouch.
  21. Place the bubble pouch into the specimen kit box. 
  22. Place the specimen kit box into the refrigerator.
  23. Repeat steps 1-22 for the next collection day. 
    1. You will collect 2 tubes a day for 2 consecutive days. 
    2. You should collect a total of 4 gray-top tubes. 

back to top

Requisition & Payment

  • Your kit has been prepaid. You DO NOT need to pay anything extra.
  • Instructions on how to fill out your requisition can be found HERE.
    • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.

back to top


  • To prepare your samples for shipping, 
    • Place the 4 gray-top tubes into the white tube holder.
    • Place the tube holder into the biohazard ziplock bag and seal.
    • Place the biohazard bag into the bubble pouch.
    • Place the bubble pouch into the specimen kit box. 
    • Place the completed test requisition form into the specimen kit box. 
    • Place the specimen kit box into the FedEx Pak.
    • Seal the FedEx Pak.
  • Make a note of the tracking number so that you can track your samples back to the lab.
    • The receipt enclosed with your FedEx pack will contain the tracking number.
  • Ship your samples as soon as possible after collection.
  • Ship your samples using the provided, prepaid FedEx Pak.
    • Take to your nearest FedEx location to ship. Visit to find your nearest location.
    • DO NOT place your samples in a FedEx drop box.

back to top


  • Results should be released to your provider 10 business days after samples are received at the lab. We cannot guarantee processing time. 
  • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 

back to top

More Resources

back to top


For general questions, email Rupa Health at For test-specific questions, contact IGeneX by calling 800-832-3200 or emailing customer support at