Test Details

Lab Company: Diagnostic Solutions
Sample Type: Stool
Collection Location: At Home

The Zonulin stand-alone test measures the levels of the zonulin protein in the gastrointestinal system. This test is used to diagnose intestinal permeability/leaky gut.    

🔑 Key Details 📦 Shipping
Preparation & Timing 📊 Results
🧪 Collection Instructions 📚 More Resources
💳 Requisition & Payment
💬 Questions?

Key Details 

  • No special preparation or timing is needed. 
  • DO NOT discard the pink liquid in the specimen vial.
  • DO NOT freeze your sample.
  • Your sample will be shipped in the provided, prepaid FedEx Clinical Pak Mailer.
  • Your sample can only be shipped Monday - Friday.
  • Your sample must be received by the lab within 5 days of collection. It is ONLY VIABLE FOR 10 DAYS post-collection.
  • NOTE: Your sample must be CLEARLY labeled and arrive at the lab with a completed test requisition form for the lab to analyze it. 

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Preparation & Timing

  • Continue taking all medications unless otherwise directed by your provider.
  • This test can be taken at any time. 

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Collection Instructions

  1. Write the following information on the specimen vial. Unlabeled samples will not be analyzed by the lab.
    1. Patient's full name
    2. Patient's date of birth (on the ID number line)
    3. Date of collection
  2. If possible, urinate prior to collecting stool.
  3. Put gloves on and pass stool into the provided collection tray.
  4. Open the specimen vial. 
    1. DO NOT discard the pink liquid in the specimen vial.
  5. Using the spoon attached to the cap of the vial, spoon stool from multiple areas of the sample into the vial.
    1. Collect from at least 4 areas going left to right.
  6. Fill the specimen vial to the red “fill line” indicated on the label. 
    1. It may not be processed if there is not enough of your sample. 
  7. Carefully mix the stool and the pink fluid with the spoon attached to the cap.
  8. Replace the cap tightly and shake the vial vigorously for 30 seconds.
  9. Place the completed test requisition form into the document holder of the specimen bag.
  10. Place the capped specimen vial into the specimen bag along with the absorbent pad.
  11. Seal the specimen bag.
  12. Place the specimen bag into the kit box.
  13. Ensure the test requisition form is completed with your personal and sample collection information. 
    1. Instructions on how to fill out your requisition can be found HERE.
    2. NOTE: Your sample will not be analyzed by the lab if it arrives at the lab without a completed test requisition form. 
  14. Place the completed test requisition form into the kit box. 
  15. Place the kit box into the provided FedEx Clinical Pak Mailer.

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Requisition & Payment

  • Your kit has been prepaid. You DO NOT need to pay anything extra.
  • Instructions on how to fill out your requisition can be found HERE.
    • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.
  • NOTE: Your sample will not be analyzed by the lab if your requisition form is not completed and sent with your sample

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  • Your sample may only be shipped Monday through Friday
  • Your sample must be received by the lab within 5 days of collection.
    • If you cannot ship the specimen on the day of collection, refrigerate the sample (DO NOT freeze) and ship within 3 days.
  • To prepare your sample for shipping,
    • Place the capped specimen vial into the specimen bag along with the absorbent pad. 
    • Place the completed test requisition form into the document holder of the specimen bag.
    • Seal the specimen bag.
    • Place the specimen bag into the test kit box.
    • Place the test kit box into the provided FedEx Clinical Pak Mailer.
  • Make a note of your tracking number (found on the Return Label) so you can track your sample back to the lab.
  • To schedule a FedEx pickup, call 1-800-463-3339
    • When the automated greeting begins, say, “Schedule a pickup.”
    • When asked if your label has the word “Stamp” on it, say, “Yes.”
    • Let them know you are shipping using a Billable Stamp.
    • Your tracking number can be found on the Return Label.

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  • Results should be released to your provider 5-10 business days after your sample is received at the lab. We cannot guarantee processing time. 
  • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 

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More Resources

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For general questions, email Rupa Health at support@rupahealth.com.  For test-specific questions, contact Diagnostic Solutions by calling 1-877-485-5336 or chatting with support