Test Details
Lab Company: Precision Analytical
Sample Type: Urine and Saliva
Collection Location: At Home
The DUTCH Plus™ is a comprehensive test that assesses your sex and adrenal hormones and their metabolites. It also looks at your Cortisol Awakening Response (CAR). This test can help you and your healthcare provider understand how your hormones are functioning and how they may be impacting your overall health.
Key Details
You must avoid certain foods, beverages, and activities before and during the collection period.
If you are taking hormones or supplements, consult the information below and your healthcare provider to discuss collection timing.
If you are a cycling woman, you must collect the samples on specific days of your cycle.
Keep your saliva samples frozen until you ship them.
Ensure the urine samples are fully dried before shipping them back.
Samples should be shipped to the lab as soon as possible after sample collection.
You MUST send the completed test requisition form and waiver (NY & RI patients only) back to the lab with your samples. Failure to send these forms with your samples may result in a recollection.
Preparation & Timing
Some supplements may affect your organic acid results. Please consult your healthcare provider regarding collection preparation and timing if you take any of the following supplements:
Tyrosine
L-Dopa
D,L-Phenylalanine (DLPA)
Mucuna
Quercetin
DO NOT skip doses of birth control unless instructed by your healthcare provider.
DO NOT collect your sample while traveling for short periods of time.
If you are taking
Patch, Pellet, or Injectable Hormones: Collect your sample midway between doses.
Sublingual Hormones: Please review the video instructions HERE.
Oral Hydrocortisone: Please review the video instructions HERE.
Glucocorticoids: Check with your healthcare provider regarding when you should collect your samples.
Men and menopausal women can collect their samples at any time.
Cycling women should take the test according to the following schedule:
Irregular cycles: Watch THIS video to determine when to collect.
Regular cycles: Use the table below to determine when to collect your sample.
You should begin collecting your sample 6-9 days before your cycle ends.
72 hours prior to sample collection
Avoid taking the following oral hormones:
Estrogen
Pregnenolone
Testosterone
48 hours prior to sample collection
Avoid the following foods before sample collection.
Avocados
Bananas
Fava beans
Avoid taking oral DHEA.
Night before Collection Day
Avoid the following beverages.
Alcohol
Caffeine
Limit your fluid intake.
If you take oral progesterone, take it at bedtime.
Sample Collection Day
Limit your fluid intake.
Keep fluids to no more than 40 oz total (spread evenly throughout the day).
Avoid the following beverages.
Alcohol
Caffeine
Avoid the following activities.
Exercise
Flossing your teeth
DO NOT eat or drink until after collection 3.
DO NOT brush your teeth in the morning until after collection 3.
DO NOT brush your teeth in the evening until after collection 6.
Collection Instructions
Collection Schedule
Collect at your first waking sleep disturbance.
Collection Number | Sample Type | Collection | Collection |
1 | Saliva | Immediately upon waking | MUST be completed within 5 minutes of waking. |
1 | Urine | Immediately upon waking | Set a timer for 30 minutes and 60 minutes. |
2 | Saliva | 30 min after waking | - |
3 | Saliva | 60 min after waking | You can now brush your teeth and eat. |
4 | Urine | 2-3 hours after waking | - |
5 | Saliva | Before dinner | - |
5 | Urine | Before dinner | - |
6 | Saliva | At bedtime | - |
6 | Urine | At bedtime | You can now brush your teeth. |
Overnight Sample | Saliva | Collect at your first waking sleep disturbance. | - |
Saliva Collection
Set the following items next to your bed the night prior to your collection.
Saliva collection device
Requisition form
Pen
Collect the first sample immediately upon waking, before getting out of bed.
Remove the tube cap.
Place the cotton swab from the collection device in your mouth.
Touch the swab as little as possible with your hands.
Leave the swab in your mouth until fully saturated.
DO NOT leave it in longer than 5 minutes.
Lightly chewing on the swab may help stimulate saliva flow.
Once the swab is saturated, place the swab back into the same tube as you found it.
DO NOT remove the inner tube.
DO NOT spit into the tube.
Complete all information on the tube label.
Freeze the saliva sample until ready to ship.
Set a timer for the second and third collections after you have completed the first.
Move to a well-lit location after the first saliva collection.
Repeat steps 3-8 for each collection.
Urine Collection
Write the following information on the collection device.
Your full name
Date of collection
Time of collection
Day of your cycle (if applicable)
Sample number (following the schedule above)
Collect your urine by either:
Urinating directly on the filter paper
Collecting your urine in a clean cup
If you collected your urine in a clean cup, dip the filter paper in the urine.
Ensure the filter paper is fully saturated.
Leave the collection device open to dry for at least 24 hours.
DO NOT touch the filter paper with your fingers.
Repeat steps 1-5 for each collection.
Once dry, close and fold each collection device.
COMPLETE THE TEST REQUISITION FORM.
Instructions on how to fill out your requisition can be found HERE.
If you live in New York or Rhode Island, please also complete the included waiver (instructions HERE).
Failure to complete and send the test requisition form may result in a sample recollection.
Place all devices in the resealable plastic bag.
Requisition & Payment
Your kit has been prepaid. You DO NOT need to pay anything extra.
Instructions on how to fill out your requisition can be found HERE.
Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.
If you live in New York or Rhode Island, please also complete the included waiver (instructions HERE) and send it to the lab with your samples.
Shipping
Please ship your samples as soon as possible after collection.
If you have to wait to ship your samples, please keep the samples in the freezer until you are ready to ship.
To prepare your samples for shipping,
Once the urine collection devices are dry, close and fold each device.
Ensure all collection devices and tubes are clearly and properly labeled.
Place all sample collection devices into the resealable plastic bag.
Place the plastic bag into the test kit box.
Place the frozen saliva tubes into the test kit box.
Place the test kit box into the provided UPS envelope.
Place the completed requisition form into the provided UPS envelope.
Place the completed waiver (NY & RI patients only) into the provided UPS envelope.
Seal the envelope.
Make a note of the tracking number so that you can track your sample back to the lab.
Results
Results should be released to your provider 7-10 business days after samples are received at the lab. Rupa cannot guarantee processing time.
Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together.
More Resources
Test Information
Collection Instructions
Questions?
For general questions, email Rupa Health at support@rupahealth.com. For test-specific questions, contact Precision Analytical by calling 1-503-687-2050 or chatting with support.