Vitamin D Test - Blood Spot
Updated over a week ago

Test Details

Lab Company: Mosaic Diagnostics
โ€‹ Sample Type: Blood Spot
โ€‹ Collection Location: At Home

The Vitamin D test measures the levels of Vitamin D circulating in your blood. Vitamin D plays an important role in a variety of health conditions.

Key Details

  • Talk to your healthcare provider if you are taking blood-thinning medications or have a blood clotting disorder.

  • Drink at least 16 oz of water a few hours prior to collection.

  • Keep your collection card cool and dry until shipping.

  • Ship your sample Monday - Thursday only.

  • DO NOT register your kit online.

Preparation & Timing

  • If your practitioner has ordered Mosaic Diagnostics tests with multiple sample types, consult THIS DOCUMENT to determine the order in which you should collect your samples.

  • Talk to your healthcare provider if you are taking blood-thinning medications or have a blood clotting disorder.

  • A few hours prior to collection:

    • Drink at least 16 oz of water.

Collection Instructions

  1. Write the following information on the barcode stickers.

    1. Your full name

    2. Date of collection

    3. Time of collection

  2. Place a barcode sticker on the collection card.

  3. Open the collection card.

    1. DO NOT touch the collection circles.

  4. Wash your hands with soap and warm water for at least 2 minutes.

  5. Dry your hands with a clean towel.

  6. Choose the finger you will prick.

    1. It is recommended to choose the middle or ring finger on your non-dominant hand.

  7. Stimulate blood flow by shaking your hand for 15-20 seconds and rubbing your chosen finger.

  8. Open the lancet by twisting the yellow tab and pulling it straight out.

  9. Wipe your finger with the antiseptic wipe.

  10. Set the antiseptic wipe aside. DO NOT throw it away yet.

  11. Press the lancet against the side of your fingertip until you hear a click.

  12. Wipe off the first drop of blood with the antiseptic wipe.

  13. Place a large, free-flowing drop of blood on each circle on the card.

    1. DO NOT touch the card with your finger.

    2. Gently massage the entire finger to assist in forming blood drops.

  14. If the circle is not filled and saturated completely, place additional drops immediately on the same circle.

    1. DO NOT add blood once the card is dry.

    2. Each spot should look like the "Acceptable" picture below.

  15. Fill all circles completely.

  16. If you have trouble producing enough blood, you can repeat steps 8-14 with the second lancet on a different finger.

  17. Once all of the circles are completely filled, wipe your finger with the sterile gauze pad.

  18. If your finger continues to bleed, hold your hand above shoulder height for about a minute until your finger stops bleeding.

  19. Bandage your finger.

  20. Bend the card on the dotted line and place it in a cool, dry, clean area.

    1. DO NOT touch the blood spots.

    2. DO NOT let the blood spots touch any surface while drying.

    3. DO NOT put the card in sunlight or near a heat source.

  21. Let the card air dry at room temperature for at least 3 hours.

  22. Throw away the lancet, antiseptic wipe, and any other materials used.

  23. Complete the test requisition form following the directions HERE.

    1. DO NOT register your kit online.

  24. Once the card is completely dry, close the flap.

  25. Place the card into the ziplock bag with the desiccant pack and seal.

Requisition & Payment

  • Your kit has been prepaid. You DO NOT need to pay anything extra.

  • Instructions on how to fill out your requisition can be found HERE.

    • Since you pre-paid for your tests, you do not need to fill in the payment information on this form.

    • DO NOT register your kit online.


  • Ship your sample Monday - Thursday only.

    • Keep the card cool and dry until shipping.

  • To prepare the sample for shipping,

    • Once the card is dry, close the flap on the card.

    • Place the collection card into the ziplock bag with the desiccant pack.

    • Seal the bag.

    • Place the ziplock bag into the provided UPS return envelope.

    • Place the completed test requisition form into the UPS return envelope.

    • Seal the UPS return envelope.

  • Make a note of the tracking number on the label so that you can track your sample back to the lab.

  • Ship using UPS.

    • You can take your sample to the nearest UPS location.

    • DO NOT place your sample in a UPS drop box.

    • You can also schedule a UPS pickup by calling 1 (800) 742-5877.


  • Results should be released to your provider 2-3 weeks after your sample is received at the lab. We cannot guarantee processing time.

  • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together.

More Resources


For general questions, email Rupa Health at For test-specific questions, contact Mosaic Diagnostics by calling 1-800-288-0383 or emailing

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