Test Details

Lab Company: ZRT Laboratory
Sample Type: Saliva
Collection Location: At Home

This test measures the levels of key hormones in your body to assess your hormonal health and identify any imbalances.

Key Details

If you are a menstruating woman or are taking hormones, you may need to collect your sample at a specific time.
You must avoid anti-aging/anti-wrinkle creams for 3 days before collection.
You must avoid certain medications for at least 2 hours before collection.
Collect your saliva sample upon waking.
Freeze your sample if not shipping within 24 hours of collection.
You will only use ONE tube.

If you are a man, child, or non-menstruating woman, you can collect your sample at any time.
If you are a menstruating woman,
- Regular cycles: Collect on Day 19, 20, or 21 of your cycle (where Day 1 is the first day of your period.)
- Irregular cycles: Collect 5 days before your period starts.
Talk to your healthcare provider if you are taking any of these medicines. They may affect test results.
- Hormone troches
- Sublingual hormones
- Steroids
Collect your sample halfway between doses of these medications.
- Hormone patches
- Vaginal rings
- Hormone pellets
- Hormone injectables

DO NOT apply topical hormones with your bare hands.
- Use latex gloves, an applicator, or a hormone-loaded syringe.
DO NOT apply topical hormones on your face, lips, and neck.

If you use topical hormones, change your sheets, pillowcases, and hand towels the night before collection.
DO NOT touch any surfaces that may be contaminated with topical hormones.
Take any night oral or topical hormones at least 12 hours before sample collection.

Avoid the following activities for at least 2 hours before collection.
- Eating
- Drinking anything but water
- Brushing your teeth
Collect your saliva sample within 30 minutes of waking.

Collect your saliva sample within 30 minutes of waking for the day.
1. Use the large tube.
Wash your hands with soap and water.
Use a clean towel to dry your hands.
Rinse your mouth with water.
Wait 5 minutes before beginning collection.
Allow saliva to pool in your mouth and then transfer it to the tube.
1. Smelling food, yawning, or pressing the tip of your tongue against your teeth can help with saliva flow.
Fill the tube at least half full, not including bubbles.
1. Collection typically takes 15-30 minutes.
2. *NOTE* If you make a mistake collecting your sample, rinse the collection tube out with hot water and no soap, shake out the remaining water, and let air dry. Re-collect at the appropriate time the next day.
Cap the tube securely.
Write the following information on the tube.
1. Patient's full name
2. Date of collection
3. Time of collection
Place the tube back into the bag.
Place the sample tube and completed test requisition form into the test kit box.
Place the test kit box inside the provided, prepaid return envelope.

Your kit has been prepaid. You DO NOT need to pay anything extra.
You must complete your requisition form and send it back with your samples.
- Since you pre-paid for your tests, you do not need to fill in the payment information on this form.

Freeze your samples if you are not shipping within 24 hours of collection.
To prepare your samples for shipping,
- Place the sample tube into the bag.
- Place the sample tube bag back into the test kit box.
- Place the completed test requisition form back into the test kit box.
- Place the test kit box into the provided, prepaid return envelope.
- Affix the prepaid label or postage.
Make a note of your return tracking number so that you can track your samples back to the lab.

Results should be released to your provider 3-5 business days after samples are received at the lab. Rupa Health cannot guarantee processing time.

Email Rupa Health at [email protected] or reach out via the chat in your patient portal.