Test Details
Lab Company: Genova Diagnostics
Sample Type: Saliva
Collection Location: At Home
This test evaluates your body's production of melatonin, a key hormone regulating sleep, by measuring its levels at three distinct times during the day.
Key Details
🧪 You will collect three samples on the same day.
❄️ Keep your samples in the freezer until you ship them.
🚚 Ship your samples within 2 days of sample collection.
🗓️ Rupa Health recommends shipping your samples Monday - Thursday only to avoid shipment delays.
Preparation & Timing
You can collect your sample any day.
You do not need to discontinue any medications unless directed to do so by your healthcare provider.
Avoid the following activities for 1 hour before each sample collection.
Eating
Brushing your teeth
Flossing your teeth
Using mouthwash
Using lip balm
Chewing gum
Using tobacco products
Collection Instructions
You will collect three samples on the same day.
Sample 1 - 7:00 am - 9:00 am
Write the following information on Label #4.
Date of birth
Date of collection
Place the label on one GREY-TOP saliva tube.
Open the tube.
Rinse your mouth with water.
Spit the water out completely.
Fill the tube with saliva to the FILL LINE (3 mL mark) within 5 minutes of starting your collection.
Your sample should reach the FILL LINE (not including bubbles).
If bubbles are in your sample, tap the tube on a hard surface to reduce foam for an accurate measurement.
Avoid getting mucus in your sample.
If you have trouble producing enough saliva, try
Pressing your tongue against the roof of your mouth
Yawning
Thinking about your favorite food
Place the cap tightly on the tube when you have filled the tube to the 3 mL mark.
Write the following information on Label #4.
Time you started collection
Time you stopped collection
Place the tube in the biohazard bag.
Freeze the tube immediately.
Your sample should be frozen for at least 2 hours before shipping.
Your sample must remain frozen until they are shipped.
Sample 2 - 3:00 pm - 5:00 pm
Write the following information on Label #6.
Date of birth
Date of collection
Place the label on one GREY-TOP saliva tube.
Open the tube.
Rinse your mouth with water.
Spit the water out completely.
Fill the tube with saliva to the FILL LINE (3 mL mark) within 5 minutes of starting your collection.
Your sample should reach the FILL LINE (not including bubbles).
If bubbles are in your sample, tap the tube on a hard surface to reduce foam for an accurate measurement.
Avoid getting mucus in your sample.
If you have trouble producing enough saliva, try
Pressing your tongue against the roof of your mouth
Yawning
Thinking about your favorite food
Place the cap tightly on the tube when you have filled the tube to the 3 mL mark.
Write the following information on Label #6.
Time you started collection
Time you stopped collection
Place the tube in the biohazard bag.
Freeze the tube immediately.
Your sample should be frozen for at least 2 hours before shipping.
Your sample must remain frozen until they are shipped.
Sample 3 - 2:30 am - 3:30 am
Before you go to bed,
Place a glass of water beside your bed.
Place one GREY-TOP tube beside your bed.
Write the following information on Label #8.
Date of birth
Date of collection
Place the label on the tube.
DO NOT TURN ON A BRIGHT LIGHT WHEN YOU WAKE UP TO COLLECT. USE LOW-LEVEL LIGHT ONLY.
Bright lights can affect your melatonin levels and lead to incorrect results.
When you wake up to collect, open the saliva tube.
Rinse your mouth with water.
Spit the water out completely.
Fill the tube with saliva to the FILL LINE (3 mL mark) within 5 minutes of starting your collection.
Your sample should reach the FILL LINE (not including bubbles).
If bubbles are in your sample, tap the tube on a hard surface to reduce foam for an accurate measurement.
Avoid getting mucus in your sample.
If you have trouble producing enough saliva, try
Pressing your tongue against the roof of your mouth
Yawning
Thinking about your favorite food
Place the cap tightly on the tube when you have filled the tube to the 3 mL mark.
Write the following information on Label #4.
Time you started collection
Time you stopped collection
Place the tube in the biohazard bag.
Freeze the tube immediately.
Your sample should be frozen for at least 2 hours before shipping.
Your sample must remain frozen until they are shipped.
In the morning, complete the test registration.
Please ensure that the name on the registration matches the name on your Rupa order EXACTLY. If it does not match, you may need to recollect your samples.
Requisition & Payment
Your kit has been prepaid. You DO NOT need to pay anything extra.
You must complete your test registration and return your activation card with your samples.
Since you pre-paid for your tests, you do not need to fill in the payment information on this form.
Shipping
Ship your samples within 2 days of collection.
Rupa Health recommends shipping Monday - Thursday only to avoid shipment delays.
To prepare your samples for shipping,
Place the sample tubes in the freezer immediately after collection.
Leave your samples in the freezer for at least 2 hours.
When ready to ship, remove your sample tubes from the freezer.
Place the sample tubes into the biohazard bag.
Place the biohazard bag into the test kit box.
Place the freezer brick into the test kit box.
Place the completed activation card into the test kit box.
Place the test kit box into the provided FedEx Clinical Pak.
Make a note of the return tracking number so you can track your sample back to the lab.
Ship your samples via FedEx.
You can take your samples to the nearest FedEx location.
DO NOT place your samples in a drop box.
You can schedule a pickup by calling FedEx at 1-800-463-3339 or visiting www.fedex.com.
Results
Results should be released to your provider 7-21 business days after samples are received at the lab. Rupa Health cannot guarantee processing time.
More Resources
Questions?
Reach out to Rupa Health via the chat in your patient portal.