Hormones Comprehensive Profile

Test Details

Lab Company: The Great Plains Laboratory
Sample Type: Saliva
Collection Location: At Home

The Hormones Comprehensive Profile provides a thorough assessment of hormonal status.

🔑 Key Details 📦 Shipping
Preparation & Timing 📊 Results
🧪 Collection Instructions 📚 More Resources
💳 Requisition & Payment
💬 Questions?

Key Details 

  • Certain foods and medications should be avoided on the day of sample collection. 
  • If you take hormones, follow the timing directions below.
  • Ship your samples as soon as possible after collection, Monday - Thursday only.
  • The samples and the provided ice pack should be frozen prior to shipping. 
  • DO NOT fill out any information on gplforms.com.

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Preparation & Timing

  • If you are a menstruating woman, collect one day during days 19-23 of your menstrual cycle (assuming a 28-day cycle).
    • Day 1 of your cycle is the first day of your period. 
  • If you are a man or non-menstruating woman, collect on any day.
  • If you are taking hormones, follow the directions below
  • The day of collection 
    • Avoid supplements and non-essential medications. 
    • Avoid caffeine-containing foods and beverages. 
    • Avoid anti-aging creams. 
    • Collect your first sample 30 minutes after waking. 
  • Avoid the following actions for 1 hour prior to collecting each sample.
    • Eating
    • Drinking
  • Avoid the following actions for 30 minutes prior to collecting each sample. 
    • Brushing your teeth
    • Flossing your teeth
    • Having dental work done 


  • If you are taking topical hormones,
    • Your last dose prior to sample collection should be 12-24 hours before collecting your first sample.
    • DO NOT use them during the day of saliva collection.
  • If you are taking sublingual hormones or troches,
    • Your last dose prior to sample collection should be 24-36 hours before collecting your first sample.
    • Drink 2 full glasses of water (about 16 oz) after taking your last dose to clean the tissues.
    • DO NOT take them during the day of saliva collection.
  • If you are taking hormone injections, subcutaneous pellets, or transdermal patches,
    • Collect your samples at the halfway point between doses.
      • Example: 3 days after a once-a-week injection
  • If you are taking oral hormones (pills),
    • Use as prescribed on the day of collection.
  • If you are taking a cortisol supplement, glucocorticoid, or cortisol-containing medication, consult with your healthcare provider prior to collecting your samples. 
    • It is recommended to stop using these products 5 days prior to sample collection.
    • Consult with your healthcare provider prior to discontinuing any prescription medications.

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Collection Instructions

Collection Schedule 

Collection Time Tube Color
1 30 minutes after waking Pink
2 Before lunch Green
3 Before dinner Orange
4 Before bedtime (at least 1 hour after dinner) Blue

Collection Instructions

  1. You must avoid certain activities prior to sample collection (see list above).
  2. You will collect 4 samples. 
    1. Collect following the schedule HERE.
  3. Wash your hands.
  4. Rinse your mouth with water. 
  5. Wait 10 minutes before beginning collection.
  6. Collect your saliva in the appropriate tube (see schedule above).
    1. Fill the tube 3/4 full (bubbles are okay).
  7. If you have trouble producing saliva, try
    1. Yawning
    2. Thinking of your favorite food
    3. Touching your tongue to the roof of your mouth.
  8. When the tube is 3/4 full, snap the tube closed tightly.
  9. Write the following information on the tube, specimen bag, and test requisition form.
    1. Your name
    2. Date of collection
    3. Time of collection 
  10. Place the saliva tube in the freezer immediately. 
  11. Repeat steps 3-9 for each of the four collections.
  12. When all four tubes have been collected, place them in the plastic bag with the absorbent material. 
  13. Keep all saliva tubes in the freezer until you are ready to ship them.
    1. Samples should be frozen for at least 4-6 hours prior to shipping.

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Requisition & Payment

  • Your kit has been prepaid. You DO NOT need to pay anything extra.
  • Instructions on how to fill out your requisition can be found HERE.
    • Since you pre-paid for your tests, you do not need to fill in the payment information on any of these forms.
    • DO NOT fill out any information on gplforms.com.

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  • Ship as soon as possible after collection, Monday - Thursday only.
  • Freeze the ice pack and your samples for at least 4-6 hours prior to shipping. 
  • To prepare your samples for shipping, 
    • Remove the saliva samples from the freezer.
    • Place the saliva samples into the biohazard bag.
    • Seal the bag.
    • Place the frozen ice pack into the silver thermo bag.
    • Place the biohazard bag into the silver thermo bag.
    • Seal the bag.
    • Place the silver thermo bag into the box.
    • Place the completed test requisition form into the box. 
    • Place the box into the FedEx Clinical Pak.
    • Fill in your information on the Billable Stamp and place it on the FedEx Clinical Pak. 
      • Keep the customer receipt.
  • Make a note of your tracking number so that you can track the sample back to the lab.
  • Ship using FedEx. 
    • You can take your samples to the nearest FedEx location.
    • DO NOT place your samples in a FedEx drop box. 
    • You can also schedule a FedEx pickup by calling 1-800-463-3339
      • At the automated greeting, say, "schedule a pickup."
      • At the next prompt, say, "schedule a pickup with a label or stamp." 
      • You will be asked if the waybill says "stamp" - say, "yes."
      • Then give your address information. 

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  • Results should be released to your provider 7-21 business days after samples are received at the lab. We cannot guarantee processing time. 
  • Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together. 
  • Your provider may choose to release your results to you. Rupa cannot release your results without provider authorization.

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More Resources

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For general questions, email Rupa Health at support@rupahealth.com. For test-specific questions, contact The Great Plains Laboratory by calling 1-800-288-0383 or chatting with support