Test Details

Lab Company: Genova Diagnostics
Sample Type: Saliva
Collection Location: At Home

The Rhythm™ test provides a comprehensive assessment of estradiol, progesterone, and testosterone spanning a full 28 days.

Key Details

You must take this test on specific days of your menstrual cycle.
If you have recently taken or are taking certain medications (see list below), consult your healthcare provider prior to testing.
Rupa Health recommends shipping Monday - Thursday only to avoid shipment delays.

You must take this test on specific days of your menstrual cycle.
- Your first sample will be collected on Day 3 of your cycle.
  - Day 1 is the day your period begins.
- If you no longer have a period, you may start the collection on any day.
If you have taken any of the following medications in the last 12 months, consult your healthcare provider before taking this test. These medications may produce artificially high results.
- Transdermal (cream) hormones
- Sublingual hormones
If you have taken any of the following drugs, herbs, or supplements in the last 3 months, consult your healthcare provider prior to taking this test. These medications may affect results.
- Melatonin (dosages >5 mg)
- Ketoconazole
- Cimetidine (Tagamet)
- Anastrozole (Arimidex)
- Letrozole (Femara)
- Exemestane (Aromasin)
- Chrysin
- Apigenin
- Tribulus terrestris
Avoid the following activities for 1 hour prior to each collection:
- Eating
- Drinking anything except water
- Brushing your teeth
- Flossing your teeth
- Using mouthwash
- Chewing gum
- Using tobacco products

You will collect samples over 11 days of your menstrual cycle. Remember, Day 1 is the day your period begins.

Rinse your mouth with drinking water.
Spit the water out completely.
Fill the collection tube with saliva until you reach the 3-ml mark.
1. You should finish the collection within 5 minutes of starting.
2. Your sample should not contain bubbles or mucus.
Replace the cap tightly.
Write the following information on the appropriate label (ie: label 1 for collection 1). Attach the label to the collection tube.
1. Your first and last name - Please ensure that the name on the label matches the name on your Rupa order EXACTLY. If it does not match, you may need to recollect your samples.
2. Time of collection
3. Date of collection
Place the tube into the biohazard bag.
Freeze the tube (in the bag) immediately.
1. Samples should be frozen a minimum of 2 hours prior to shipping.
2. Keep samples frozen until ready to ship.
Repeat steps 1-7 for each collection (schedule HERE).
Complete the test registration (instructions HERE).
1. Please ensure that the name on the registration matches the name on your Rupa order EXACTLY. If it does not match, you may need to recollect your samples.
When you are ready to ship the samples, place the frozen samples into the biohazard bag.
Place the biohazard bag into the test kit box.
Place the test kit box and completed activation card into the provided, prepaid mailing envelope.

Your kit has been prepaid. You DO NOT need to pay anything extra.
Instructions on how to complete your test registration can be found HERE.
- Since you pre-paid for your tests, you do not need to fill in the payment information on this form.

Ship your samples as soon as possible after the final collection.
Rupa Health recommends shipping Monday - Thursday only to avoid shipment delays.
To prepare your samples for shipping,
- Place the frozen sample tubes into the biohazard bag.
- Place the biohazard bag into the test kit box.
- Place the completed activation card into the test kit box.
- Place the test kit box into the provided return mailer.
Make a note of the tracking number on the label so that you can track your samples back to the lab.
Ship your samples via FedEx.
- You can take your samples to the nearest FedEx location.
- DO NOT place your samples in a drop box.
- You can also schedule a FedEx pickup by calling 1-800-463-3339 or visiting their website www.fedex.com.

Results should be released to your provider 7-21 business days after samples are received at the lab. We cannot guarantee processing time.
Your provider will notify you when your results are in and schedule a follow-up appointment for you to review them together.

For general questions, email Rupa Health at support@rupahealth.com. For test-specific questions, contact Genova Diagnostics by calling 800-522-4762 or chatting with support.